LKB 1 is a master kinase that activates 13 protein kinases of the AMPK subfamily , including the MARK / PAR - 1 kinases

Materials and Methods. Materials. Protease-inhibitor cocktail tablets and sequencing grade trypsin were obtained from Roche. N-octyl-glucoside was from Calbiochem, and tissue culture reagents were from Life Technologies. Tetracyclin-free foetal bovine sera (FBS) was from Perbio, and other tissue culture reagents were from Biowhittaker. Precast 4–12% and 10% polyacrylamide Bis-Tris gels were obtained from Invitrogen; P81 phosphocellulose paper was from Whatman; Phenformin from Sigma and AICA riboside from TorontoXX. [γP]ATP, glutathione-Sepharose, Protein G-Sepharose were purchased from Amersham Biosciences. All peptides were synthesised by Dr Graham Bloomberg at the University of Bristol.